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The Arabidopsis Embryo Mutant schlepperless Has a Defect in the Chaperonin-60α Gene

Authors
Publisher
American Society of Plant Physiologists
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

The Arabidopsis Embryo Mutant schlepperless Has a Defect in the Chaperonin-60α Gene by Nestor R. Apuya, Ramin Yadegari, Robert L. Fischer, John J. Harada, J. Lynn Zimmerman, and Robert B. Goldberg Plantphysiol Volume 126(2):717-730 June 1, 2001 ©2001 by American Society of Plant Biologists Morphology of schlepperless embryos. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists Developmental analysis of schlepperlessembryos. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists Germination of WT and schlepperlessseeds in vitro. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists schlepperless embryo is competent to transcribe late-maturation genes. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists The Chaperonin-60α gene is interrupted by T-DNA in slp mutant. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists Alignment of the Arabidopsis chaperonin-60α protein with other related proteins. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists RNA-blot analysis ofchaperonin-60α. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists Transmission electron microscopy analysis of plastids. Apuya N et al. Plantphysiol 2001;126:717-730 ©2001 by American Society of Plant Biologists Morphology of schlepperless embryos. Mature embryos from WT (A, B, and C) and schlepperless (D, E, and F). Whole mount photographs of embryos dissected out of the same mature silique (A and D). Sections of embryos from mature silique embedded in LR White plastic resin (B and E). Nomarski photographs of embryos taken from the same mature silique (C and F). C, Cotyledon; Ep, epidermis; G, ground tissue; H, hypocotyl; SA, shoot apical meristem; V, vascular tissue. Bars = 50 μm. Developmental analysis of schlepperlessembryos. A develop

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