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Synovial lymphoid neogenesis is associated with IL-23 expression and disease activity in rheumatoid arthritis

Authors
Journal
Journal of Translational Medicine
1479-5876
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
9
Identifiers
DOI: 10.1186/1479-5876-9-s2-p33
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

Synovial lymphoid neogenesis is associated with IL-23 expression and disease activity in rheumatoid arthritis POSTER PRESENTATION Open Access Synovial lymphoid neogenesis is associated with IL-23 expression and disease activity in rheumatoid arthritis Juan D Cañete1*, Raquel Celis1, Julio Ramírez1, Sara Marsal2, Gabriela Ávila2, Raimón Sanmartí1, José L Pablos3 From 6th European Workshop on Immune-Mediated Inflammatory Diseases Nice, France. 23-25 November 2011 Objective We analyzed whether LN is associated with specific pat- terns of inflammatory cytokine expression in synovial tis- sue (ST), and the potential association of cytokine expression with disease activity and response to therapy. Methods ST samples were obtained by arthroscopy from the inflamed knee of 62 RA patients. A second biopsy was obtained after a mean of 8±5 months of treatment in 21 patients who started TNF blockers. ST samples were immunostained with CD3 (T cell), CD20 (B cell), and MECA-79 (high endothelial vessels). Total ST mRNA was extracted and gene expression of CCR7, LT-beta, IL-7, IL- 10, IL-17A, IL-21, IL-22, IL-23, TNF-alpha, IL-1b, and IL- 6 was measured by quantitative real-time PCR. Clinical and biological data were collected at inclusion and after a median follow-up of 2.3 years. Results 28 out of 62 patients (45.2%) had LN, which was asso- ciated with a significantly-higher expression of three key molecular markers of LN: CCR7, IL-7 and LT-beta (p<0.037, p<0.038, and p=0.009, respectively). LN-positive patients also had higher IL-23 (p=0.035) and higher DAS28 score, both at inclusion and at the end of follow- up (p=0.055 and p=0.037, respectively). In the group of patients with ST before and after TNF blocker therapy, EULAR good response was associated with IL-10 reduc- tion (p=0.035). In the entire group, EULAR response cor- related with IL-1b expression (P=0.014). Conclusion RA patients with LN were characterized by higher expres- sion of key molecular markers of LN, IL-23 and higher d

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