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Intra-arterial adenoviral mediated tumor transfection in a novel model of cancer gene therapy

BioMed Central
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Abstract ral ss BioMed CentMolecular Cancer Open AcceResearch Intra-arterial adenoviral mediated tumor transfection in a novel model of cancer gene therapy Gustavo Cabrera*1, Stacy L Porvasnik2, Paul E DiCorleto3, Maria Siemionow4 and Corey K Goldman5 Address: 1Gene Therapy Laboratory, National Cancer Institute, Mexico City, Mexico, 2Powel Gene Therapy Center, The University of Florida, Gainesville, USA, 3Department of Cell Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA, 4Department of Plastic and Reconstructive Surgery, The Cleveland Clinic Foundation, Cleveland, USA and 5Department of Vascular Medicine, Ochsner Clinic Foundation, New Orleans, USA Email: Gustavo Cabrera* - [email protected]; Stacy L Porvasnik - [email protected]; Paul E DiCorleto - [email protected]; Maria Siemionow - [email protected]; Corey K Goldman - [email protected] * Corresponding author Abstract Background: The aim of the present study was to develop and characterize a novel in vivo cancer gene therapy model in which intra-arterial adenoviral gene delivery can be characterized. In this model, the rat cremaster muscle serves as the site for tumor growth and provides convenient and isolated access to the tumor parenchyma with discrete control of arterial and venous access for delivery of agents. Results: Utilizing adenovirus encoding the green fluorescent protein we demonstrated broad tumor transfection. We also observed a dose dependant increment in luciferase activity at the tumor site using an adenovirus encoding the luciferase reporter gene. Finally, we tested the intra- arterial adenovirus dwelling time required to achieve optimal tumor transfection and observed a minimum time of 30 minutes. Conclusion: We conclude that adenovirus mediated tumor transfection grown in the cremaster muscle of athymic nude rats via an intra-arterial route could be achieved. This model allows definition of the variables that affect intra-arterial tumor transfec

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