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Unusual behaviour of NADP-linked malic enzyme from crustacean tissues on 2′,5′ADP-sepharose 4B

Authors
Journal
Comparative Biochemistry and Physiology Part B Comparative Biochemistry
0305-0491
Publisher
Elsevier
Publication Date
Volume
72
Issue
4
Identifiers
DOI: 10.1016/0305-0491(82)90520-x
Disciplines
  • Biology

Abstract

Abstract 1. 1. Essential differences in the binding of NADP-dependent malic enzymes from mammals, fish and crustacea to 2′,5′ADP-Sepharose 4B have been shown. 2. 2. The enzymes from mammalian and fish tissues interact with 2′,5′ADP-Sepharose, whereas the enzymes from crayfish Orconectes limosus and shrimp Crangon crangon tissues do not. 3. 3. These results provide evidence that 2′,5′ADP-Sepharose cannot be used for the purification of NADP-dependent malic enzyme from crayfish and shrimp and indicate presumably a structural difference in the NADP-binding site of crustacean enzyme.

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