Affordable Access

Publisher Website

Direct biocatalytic one-pot-transformation of cyclohexanol with molecular oxygen into ɛ-caprolactone

Authors
Journal
Enzyme and Microbial Technology
0141-0229
Publisher
Elsevier
Publication Date
Volume
53
Issue
4
Identifiers
DOI: 10.1016/j.enzmictec.2013.03.011
Keywords
  • Alcohol Dehydrogenase
  • Baeyer–Villiger Monooxygenase
  • Cyclohexanol
  • Cyclohexanone
  • ɛ-Caprolactone
  • Oxidation

Abstract

Abstract The development of a biocatalytic process concept for ɛ-caprolactone, which directly converts cyclohexanol as an easily available industrial raw material into the desired ɛ-caprolactone in a one-pot fashion while only requiring air as sole reagent, is reported. The desired product ɛ-caprolactone was obtained with 94–97% conversion when operating at a substrate concentration in the range of 20–60mM. At higher substrate concentrations, however, a significant drop of conversion was found. Subsequent detailed studies on the impact of the starting material, intermediate and product components revealed a significant inhibition and partial deactivation of the BVMO by the product ɛ-caprolactone (in particular at higher concentrations) as well as an inhibition of the BVMO by cyclohexanol and cyclohexanone.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments