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Localization of α-bungarotoxin binding sites in synapses of the developing chick retina

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
201
Issue
1
Identifiers
DOI: 10.1016/0006-8993(80)90774-x
Keywords
  • α-Bungarotoxin
  • Chick Retina
  • Cholinergic Receptors
  • Amacrine Cell

Abstract

Abstract Alpha-bungarotoxin binding sites in chick retina from 12 days in ovo to hatching, were visualized at the ultrastructural level by use of a 1:1 conjugate of horseradish peroxidase with α-bungarotoxin. At all stages binding sites in the inner plexiform layer were localized in synapses, predominantly on or near the postsynaptic membrane. Localization of binding sites was found in bipolar and amacrine-cell synapses which appeared morphologically immature as well as more well developed synapses. The results suggest that α-bungarotoxin-binding synapses, tentatively considered to be nicotinic cholinergic, are formed throughout the course of synaptogenesis, and that the aggregation of nicotinic receptors occurs early in the formation of these synapses.

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