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A flow-through microsampling device applied to an ion-exchange chromatography system

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
27
Issue
3
Identifiers
DOI: 10.1016/0165-022x(93)90002-6
Keywords
  • Piezo-Electric Drop Generator
  • Flow-Through Liquid Sampler
  • Fused Rocket Electrophoresis

Abstract

Abstract A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0–9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

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