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Molecular Mechanism of the ATP Synthase's FoMotor Probed by Mutational Analyses of Subunit a

Authors
Journal
Journal of Molecular Biology
0022-2836
Publisher
Elsevier
Publication Date
Volume
322
Issue
2
Identifiers
DOI: 10.1016/s0022-2836(02)00731-3
Keywords
  • Na+-Translocating Atp Synthase
  • Fomotor
  • Coupling Mechanism
  • Subunit A
Disciplines
  • Biology

Abstract

Abstract The most prominent residue of subunit a of the F 1F o ATP synthase is a universally conserved arginine (aR227 in Propionigenium modestum), which was reported to permit no substitution with retention of ATP synthesis or H +-coupled ATP hydrolysis activity. We show here that ATP synthases with R227K or R227H mutations in the P. modestum a subunit catalyse ATP-driven Na + transport above or below pH 8.0, respectively. Reconstituted F o with either mutation catalysed 22Na + out/Na + in exchange with similar pH profiles as found in ATP-driven Na + transport. ATP synthase with an aR227A substitution catalysed Na +-dependent ATP hydrolysis, which was completely inhibited by dicyclohexylcarbodiimide, but not coupled to Na + transport. This suggests that in the mutant the dissociation of Na + becomes more difficult and that the alkali ions remain therefore permanently bound to the c subunit sites. The reconstituted mutant enzyme was also able to synthesise ATP in the presence of a membrane potential, which stopped at elevated external Na + concentrations. These observations reinforce the importance of aR227 to facilitate the dissociation of Na + from approaching rotor sites. This task of aR227 was corroborated by other results with the aR227A mutant: (i) after reconstitution into liposomes, F o with the aR227A mutation did not catalyse 22Na + out/Na + in exchange at high internal sodium concentrations, and (ii) at a constant ΔpNa +, 22Na + uptake was inhibited at elevated internal Na + concentrations. Hence, in mutant aR227A, sodium ions can only dissociate from their rotor sites into a reservoir of low sodium ion concentration, whereas in the wild-type the positively charged aR227 allows the dissociation of Na + even into compartments of high Na + concentration.

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