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Visualization of immunosorption by scanning electron microscopy

Authors
Journal
Veterinary Immunology and Immunopathology
0165-2427
Publisher
Elsevier
Publication Date
Volume
1
Issue
3
Identifiers
DOI: 10.1016/0165-2427(80)90027-6
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract Immunosorption has become a very important biochemical and serological tool and it is the purpose of this paper to visualize this process qualitatively using the scanning electron microscope. Different carriers (i.e. CNBr activated cellulose and Sepharose, glutaraldehyde treated acrylamide-agarose beads Magnogel, and polystyrene cover slips) were coated with different antibodies and incubated with their homologous antigens such as pneumococci, ferritin, polymeric Salmonella flagellin and mechanically detached flagella, as well as tetanus toxoid, E. coli bacteriophage T 4 and Rota virus particles. As negative controls the sorbents were incubated with heterologous antigens.

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