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In vitro maturation of caprine oocytes in different culture media

Authors
Journal
Small Ruminant Research
0921-4488
Publisher
Elsevier
Publication Date
Volume
47
Issue
2
Identifiers
DOI: 10.1016/s0921-4488(02)00238-9
Keywords
  • Caprine
  • Oocyte Maturation
  • Egs
  • Ess
  • Fbs
Disciplines
  • Biology

Abstract

Abstract Oocytes were aspirated from caprine ovaries, washed and cultured in TCM-199 containing penicillin, streptomycin and 10, 15, or 20% of fetal bovine serum (FBS), estrous sheep serum (ESS) or estrous goat serum (EGS). After 24–26 h culture, oocytes were separated from cumulus and corona cells by hyaluronidase and by passing through a fine pipette, fixed in acetic alcohol, stained with aceto orcein and observed under a phase-contrast microscope for evidence of maturation. High maturation rates (74–94%) were observed in all concentrations of the three different sera examined. No significant difference was observed between different concentrations and among different sera. Almost no maturation (4%) was observed in the medium lacking protein supplement. In conclusion, these sera with the concentrations examined can be substituted for one another for in vitro maturation of caprine oocytes.

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