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Effects of hormones on post-implantation mouse embryos in vitro. I. Insulin

The Journal of Experimental Zoology
Wiley Blackwell (John Wiley & Sons)
Publication Date
  • Biology


Day 9 mouse embryos were cultured in 50% fetal calf serum and 50% Waymouth's medium supplemented with various amounts of insulin. Unmodified medium contained approximately 25% of the normal adult mouse blood insulin. The addition of 0.1 to 1.0 Μg insulin/ml to Waymouth's medium was most beneficial to in vitro development. There was a statistically significant increase in protein synthesis, RNA content, glucose utilization, and heart rate, as well as a significant decrease in pericardial edema. DNA, synthesis was unchanged or slightly decreased. The optimal amount of added insulin (0.05 Μg/ml) is much higher than the normal circulating levels in adult mice.

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