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Schiff base chemiluminescence with Fenton's reagent for the determination of primary amines and amino acids

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
247
Issue
1
Identifiers
DOI: 10.1016/s0003-2670(00)83048-2
Keywords
  • Chemiluminescence
  • Flow System
  • Amines
  • Amino Acids
  • Fenton'S Reagent
  • Schiff Bases

Abstract

Abstract A chemiluminescence method involving Schiff base formation was studied for the determination of primary amines and amino acids. The Schiff bases derived from phenylacetaldehyde in methanol for amines and sodium bis(2-ethylhexyl)sulphosuccinate reversed micellar solution for amino acids were found to produce strong chemiluminescence when oxidized with iron(II) and hydrogen peroxide (Fenton's reagent). The chemiluminescence signal was measured by means of a flow-injection system, 100 μl of the Schiff base sample solution being injected into a methanol carrier and then mixed with the Fenton's reagent solution. The determination limits were 1.5 × 10 −8 M for n-hexylamine and 1.4 × 10 −7 M for alanine and the linear dynamic range was 10 3–10 5 M. The chemiluminescence mechanism is discussed briefly.

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