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Biosensing based on NADH detection coupled to electrogenerated chemiluminescence from ruthenium tris(2,2′-bipyridine)

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
41
Issue
6
Identifiers
DOI: 10.1016/0039-9140(94)e0074-2

Abstract

Abstract A flow injection analysis system for a reduced form of nicotinamide adenine dinucleotide (NADH) was constructed by the electrogenerated chemiluminescence using ruthenium tris(2,2′-bipyridine) complex, and this technique was applied to the determination of ethanol concentration by using an immobilized alcohol dehydrogenase (ADH). The NADH determination was optimum under the following conditions. The flow rate was 1.5 to 2.0 ml/min, and the applied voltage was 1.6 V. The NADH calibration plot showed the linear correlation in the concentration range 10 to 250μ M, and the lower detection limit was 10μ M. Ethanol was successfully measured in the concentration range 10 ppm to 5% by using ADH-immobilized glass beads.

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