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Real-time PCR in Food Science: Current Technology and Applications

Centers for Disease Control and Prevention
Publication Date
DOI: 10.3201/eid.1908.130524
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BOOKS AND MEDIA 1352 Emerging Infectious Diseases • • Vol. 19, No. 8, August 2013 PCR Detection of Microbial Pathogens Mark Wilks, Editor Humana Press, New York, New York, USA, 2012 ISBN-10: 1603273522 ISBN-13: 978:1603273527 Pages: 328; Price: US $84.00 This book covers general chal- lenges of introducing primarily non- commercial PCRs and specific pro- cedures into the laboratory, including sample treatment, extraction proto- cols, quality and quality assurance, and internal and external laboratory processes. The chapters on specific pathogens illustrate principles that could be applied in many diagnostic laboratories. The editor’s preface to this book is helpful in framing approaches to PCR pathogen detection methods. The focus is primarily on detection of bacterial pathogens, with the ex- ception of Pneumocystis spp., and the case is made for using less expensive noncommercial strategies that enable more flexibility and customization. The book addresses the many param- eters of nucleic acid preparation, buf- fer choice, primer construction, inhi- bition, cycling parameters, detection, and statistical analysis. The ≈300 pages of text are di- vided in 21 chapters, of which the first 3 cover concepts of importance to all clinical laboratories using PCRs. The third chapter, which covers quality and quality assurance, is particularly comprehensive in its treatment of in- ternal and external laboratory process and PCR controls. This chapter covers a variety of concepts, from Westguard rules for investigations of systematic and other errors, to proficiency testing, and includes many useful tables. Of importance to clinical laboratories and epidemiologic investigations alike, the authors make an essential point that up to 75% of errors in the testing process can be attributed to improper sample collection and transport of specimens, areas that often get less at- tention than assay quality control. The fourth chapter covers p

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