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Gene expression profiling of cutaneous wound healing

BioMed Central
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Abstract ral ss BioMed CentJournal of Translational Medicine Open AcceResearch Gene expression profiling of cutaneous wound healing Kavita Deonarine1, Monica C Panelli1, Mitchell E Stashower2, Ping Jin1, Kina Smith1, Herbert B Slade3, Christopher Norwood, Ena Wang1, Francesco M Marincola1 and David F Stroncek*1 Address: 1Immunogenetics Section, Department of Transfusion Medicine, Clinical Center National Institutes of Health, Bethesda MD, 20892, USA, 2The Clinical Skin Center of Northern Virginia, Fairfax, VA, 22033, USA and 3DFB Pharmaceuticals, Fort Worth, TX, 76107, USA Email: Kavita Deonarine - [email protected]; Monica C Panelli - [email protected]; Mitchell E Stashower - [email protected]; Ping Jin - [email protected]; Kina Smith - [email protected]; Herbert B Slade - [email protected]; Christopher Norwood - [email protected]; Ena Wang - [email protected]; Francesco M Marincola - [email protected]; David F Stroncek* - [email protected] * Corresponding author Abstract Background: Although the sequence of events leading to wound repair has been described at the cellular and, to a limited extent, at the protein level this process has yet to be fully elucidated. Genome wide transcriptional analysis tools promise to further define the global picture of this complex progression of events. Study Design: This study was part of a placebo-controlled double-blind clinical trial in which basal cell carcinomas were treated topically with an immunomodifier – toll-like receptor 7 agonist: imiquimod. The fourteen patients with basal cell carcinoma in the placebo arm of the trial received placebo treatment consisting solely of vehicle cream. A skin punch biopsy was obtained immediately before treatment and at the end of the placebo treatment (after 2, 4 or 8 days). 17.5K cDNA microarrays were utilized to profile the biopsy material. Results: Four gene signatures whose expression changed relative to baseline (before wound induction by

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