Affordable Access

Publisher Website

Cellular ion route in rabbit gallbladder epithelium

Authors
Journal
Bioelectrochemistry and Bioenergetics
0302-4598
Publisher
Elsevier
Publication Date
Volume
3
Issue
1
Identifiers
DOI: 10.1016/0302-4598(76)85009-x

Abstract

Abstract The electric properties of the cells were studied in order to investigate how Na + and Cl − are transported across the gallbladder epithelium. A negative intracellular electric potential of about 60 mV and a near-zero transepithelial potential were measured. By replacing K + on the mucosal or on the serosal side, a paracellular shunt was revealed. By carrying out rapid K + changes on the mucosal side and immediate intracellular potential measurements, the mucosal potential change (Δ Um) coulb be calculated. On this basis, the relationships were obtained between lot [K +]m and Δ Um, with constant [Cl −] and [HCO 3 −] or with these anions replaced by SO 4 2− or with Na + replaced by choline. The results show that the mucosal barrier has a large conductance for K +, a modest conductance for Na + and no-conductance at all for anions. These conclusions were confirmed by the bilateral simultaneous replacement of Na + with choline or of Cl − and HCO 3 − with I − or with SO 4 2−. The intracellular potential is not affected. Thus, both the mucosal and the serosal barrier seem to exhibit similar electric properties. Since the electrical conductance by Cl − is zero through the cell and is insufficient through the shunt to account for the measured net transport of the anion, a coupled neutral transport of Na + and Cl − through the cellular pathway is the most logical explanation of these data.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

The route of passive ion movement through the epit...

on The Journal of Membrane Biolog... 1972

Transcellular ion route in rabbit gallbladder. Ele...

on Pflügers Archiv - European Jou... 1975

Ion transport across the gallbladder epithelium.

on Current drug targets. Immune,... June 2005

Ion transport across gallbladder epithelium.

on Physiological reviews April 1989
More articles like this..