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Analysis of adipimidate-crosslinked lysine

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
107
Issue
2
Identifiers
DOI: 10.1016/0003-2697(80)90389-9
Keywords
  • Protein Analyses And Reagents
Disciplines
  • Biology

Abstract

Abstract The chromatographic conditions for separation of N,N′-bislysyl(ϵ- N)adipamidine and N-lysyl(ϵ- N)adipamidinic acid, which were the products of acid hydrolysis of proteins treated with adipimidate esters, from other amino acids on an amino acid analyzer were established including their ninhydrin color values. Kinetics of decomposition of these lysine derivatives under the conditions of total acid hydrolysis of protein are also reported.

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