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Determination of primary and secondary aliphatic amines with high performance liquid chromatography based on the derivatization using 1,3,5,7-tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)-difluoroboradiaza-s-indacene

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
84
Issue
4
Identifiers
DOI: 10.1016/j.talanta.2011.03.021
Keywords
  • Dimethylamine
  • Aliphatic Amine
  • 1
  • 3
  • 5
  • 7-Tetramethyl-8-(N-Hydroxysuccinimidyl Butyric Ester)-Difluoroboradiaza-S-Indacene (Tmbb-Su)
  • Fluorescence Detection
  • High Performance Liquid Chromatography
Disciplines
  • Design

Abstract

Abstract In this article, the simultaneous determination of primary and secondary aliphatic amines including dimethylamine (DMA), diethylamine and eleven primary aliphatic amines by high performance liquid chromatography (HPLC) with fluorescence detection has been achieved using a BODIPY-based fluorescent derivatization reagent, 1,3,5,7-tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)-difluoroboradiaza-s-indacene (TMBB-Su). The derivatization reaction of TMBB-Su with aliphatic amines was optimized with orthogonal design experiment and the derivatization reaction proceeded at 15°C for 25min. The baseline separation of these derivatives was carried out on a C8 column with methanol–tetrahydrofuran-50mM pH 6.50 HAc–NaAc buffer (55/5/40, v/v/v) as a mobile phase. Detected at the excitation and emission of 490 and 510nm, respectively, the detection limits were obtained in the range of 0.01–0.04nM (signal-to-noise ratio=3). The proposed method has been applied to the determination of trace aliphatic amines in viscera samples from mice without complex pretreatment or enrichment method. The recoveries ranged from 95.1% to 106.8%, depending on the samples investigated.

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