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The measurement of serum paracetamol using a discrete analyser

Hindawi Publishing Corporation
Publication Date
DOI: 10.1155/s1463924683000358
  • Research Article
  • Biology
  • Chemistry
  • Medicine


Journal of Automatic Chemistry, Volume 5, Number 3 (July-September 1983), pages 146-149 The measurement of serum paracetamol using a discrete analyser R. Stewart Campbell, Peter M. Hammond, Michael D. Scawen and Christopher P. Price 1.Department of Clinical Biochemistry, Addenbrooke’s Hospital, Hills Road, Cambridge CB2 2QR, UK, 2. PHLS Centre for Applied Microbiology and Research, Microbial Technology Laboratory, Porton Down, Salisbury SP4 0JG, UK Introduction The current trend in clinical biochemistry towards the use of discrete analysers capable of performing a wide variety of analyses on a single sample, at the discretion of the analyst, suggests that this type ofequipment may have an important role to play in the emergency laboratory. There are, however, few analysers possessing the facility for stat discretionary testing. Recently, a specific method for the measurement of para- cetamol has been described [1]. The method is a two-stage reaction involving, firstly, the enzymatic hydrolysis ofthe parent compound to p-aminophenol and acetate; and, secondly, the specific colorimetric detection of the p-aminophenol by the formation of a blue indophenol dye [2]. This assay has been adapted for use on a microcentrifugal analyser. In performing the adaptation the main objectives were to investigate (1) the limitations imposed by a two-stage reaction sequence; and (2) the performance ofsuch an automated system. Materials and methods Reagents All the reagents used were supplied in a diagnostic kit from Cambridge Life Sciences plc of Cambridge, UK. The enzyme reagent was a lyophilized preparation containing a buffer. Two colour reagents were supplied: colour reagent A was an aqueous solution of o-cresol, and colour reagent B was an ammoniacal copper sulphate solution. The kit also contained an aqueous paracetamol solution of 2-00mmol/1 as the standard. Instrumentation The method was set up on a microcentrifugal analyser: the Multistat III (Instrumentation Laboratory, Lexington, USA). The instrument i

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