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Electroosmotic flow mobilities in open and packed capillaries with spermine and other amine flow modifiers

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
768
Issue
1
Identifiers
DOI: 10.1016/s0021-9673(96)00835-7
Keywords
  • Electroosmotic Flow
  • Buffer Composition
  • Inorganic Ion Analysis
  • Spermine
  • Amines

Abstract

Abstract The effect of some cationic amines (spermine, spermidine, hexamethonium bromide and tetramethylammonium bromide) on the mobility of electroosmotic flow (EOF) in open fused-silica capillaries was studied. The EOF was found to be significantly reduced with spermine and spermidine and to a lesser extent with hexamethonium bromide. Tetramethylammonium bromide was found to be ineffective as a flow modifier. Spermine (10–20 μm M) gave a stable reduced EOF at pH 2.50, while 400–500 μ M was required at pH 7.0. In a silica-packed capillary, higher concentrations of spermine were required to significantly change the EOF (up to 1 m M). The very low adsorption coefficients ( M − cm −1) of spermine [ ϵ 200=250, ϵ 210=35] and spermidine [ ϵ 200=118, ϵ 210=3] at low UV wavelengths makes them useful as usrface-charge modification agents when using UV detection, and their low conductivity makes them suitable for use with conductivity detection.

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