Affordable Access

Publisher Website

Detection of the Readthrough Protein of Barley Yellow Dwarf Virus

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
202
Issue
2
Identifiers
DOI: 10.1006/viro.1994.1427
Disciplines
  • Biology

Abstract

Abstract The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) raised against BYDV-PAV-IL could specifically detect the presence of the 72-kDa readthrough protein in extracts from the BYDV-infected leaf tissue. The results suggest that ORF 5 (50-kDa protein) is translated by readthrough of ORF 3 (22-kDa coat protein) to produce the 72-kDa protein. The readthrough protein is thought to be a structural protein on the external surface of BYDV.

There are no comments yet on this publication. Be the first to share your thoughts.