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A direct antigen-binding assa to screen hybridoma supernatants

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
151
Issue
1
Identifiers
DOI: 10.1016/0003-2697(85)90071-5
Disciplines
  • Biology

Abstract

Abstract A simple and convenient method of directly assaying hybridoma supernatants for the desired monoclonal antibodies is described which obviates the need for labeled second or third antibody conjugates. Culture supernatants (1–5 μl) were directly spotted onto a nitrocellulose sheet, and additional protein binding sites blocked with bovine serum albumin and incubated with enzymelabeled, radioactive, or fluorescent antigen. Positive hybridoma supernatants were identified after washing and detection of bound antigen by appropriate mens.

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