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Phylogenetic Analysis of an Anaerobic, Trichlorobenzene-Transforming Microbial Consortium

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
  • General Microbial Ecology
Disciplines
  • Archaeology

Abstract

A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869–3877, 1998).

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