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Cellobiohydrolase I (Cel7A) fromTrichoderma reeseihas chitosanase activity

Authors
Journal
Journal of Molecular Catalysis B Enzymatic
1381-1177
Publisher
Elsevier
Publication Date
Volume
47
Identifiers
DOI: 10.1016/j.molcatb.2007.05.004
Keywords
  • Trichoderma Reesei(Hypocrea Jecorina)
  • Aspergillus Aculeatus
  • Cellobiohydrolase
  • Chitosanase
  • Glycoside Hydrolase Family (Ghf) 7
Disciplines
  • Biology

Abstract

Abstract Cellobiohydrolase CBH I (Cel7A) from the filamentous fungus Trichoderma reesei ( TrCBHI), which is a member of glycoside hydrolase family (GHF) 7, was expressed in Aspergillus oryzae. We found that the recombinant enzyme showed significant chitosanase activity, as well as cellulase activity, and acted in an endo-type manner on soluble polymeric substrate. Furthermore, another GHF7 CBH I from Aspergillus aculeatus ( AaCBHI) expressed in A. oryzae also had chitosanase activity, while endoglucanase EG I (Cel7B) from T. reesei had no activity towards chitosan. To our knowledge, this is the first report of GHF7 enzymes possessing chitosanase activity.

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