Affordable Access

Polymerase chain reaction detection of Haemophilus ducreyi DNA

Authors
Journal
Sexually Transmitted Infections
1368-4973
Publisher
BMJ
Publication Date
Keywords
  • Original Articles
Disciplines
  • Medicine

Abstract

OBJECTIVES: To develop a polymerase chain reaction (PCR) method to detect Haemophilus ducreyi DNA in cultured isolates and clinical material. METHODS: Primers specific to the H ducreyi 16s rRNA gene were synthesised. PCR conditions were optimised and products were verified by restriction endonuclease digestion and agarose gel electrophoresis. RESULTS: The method was able to detect all 28 H ducreyi strains tested; specificity was demonstrated using lysates of 12 related organisms. Applied to clinical samples from genital ulcer swabs obtained in Harare, Zimbabwe, H ducreyi DNA was detected in repeated assays in 35 clinical samples. CONCLUSION: PCR amplification using primers from the 16s rRNA gene may be a useful alternative to culture for the detection of H ducreyi and the diagnosis of chancroid. 




There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Polymerase chain reaction detection of Haemophilus...

on Sexually transmitted infection... February 1998

Development of a polymerase chain reaction assay f...

on Sexually transmitted diseases 1994

Haemophilus ducreyi detection by polymerase chain...

on International Journal of STD &... April 2009

Haemophilus ducreyi detection by polymerase chain...

on International Journal of STD &... Jan 01, 2009
More articles like this..