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Determination of the molecular weight ofSaccharomyces cerevisiaenuclear DNA

Authors
Journal
Journal of Molecular Biology
0022-2836
Publisher
Elsevier
Publication Date
Volume
95
Issue
2
Identifiers
DOI: 10.1016/0022-2836(75)90397-6

Abstract

Abstract Viscoelastic retardation-time experiments on the DNA released from spheroplasts of the yeast Saccharomyces cerevisiae yield a molecular weight of 2 × 10 9 for the largest DNA, assuming linear unbranched DNA, and of 4.3 × 10 9 assuming circular unbranched DNA. Both log and stationary-phase cells give the same results. Comparison of these results with the nuclear DNA content of S. cerevisiae determined by renaturation kinetics suggests that the largest piece of DNA in the yeast nucleus may, at least during part of the cell cycle, consist of from one-fourth to all of the yeast genome.

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