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Modifications papar and thin layer chromatographic methods to increase sensitivity for detecting N-methylated indoleamines in urine.

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
30
Identifiers
DOI: 10.1016/s0021-9673(00)84125-4

Abstract

Abstract Sensitive and reproducble modifications of paper and thin layer chromatograhic methods have been developed for detecting N-methylated indoleamines in the urine. The methods consist of three main steps: (1) a preliminary concentration of urine, followed by (2) the purification of indoleamine fractions using an ion-exchange resin, and (3) identification procedures. By the present methods, free and conjugated indoleamines could be examined separately, and as little as 1 μg of indoleamines per 1000 ml urine could be consistently detected. The R F values and the color of several indoleamines and related compounds on paper and thin layer chromatograms are presented. The usefulness of these methods was proved in the analysis of schizophrenic urines, in which bufotenin, N-methylserotonin, N-dimethyltryptamine and 5-methoxy. N-dimethyltryptamine were revealed when the patients were receiving a monoamine oxidase inhibitor.

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