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Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics

Authors
Journal
BMC Developmental Biology
1471-213X
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
8
Issue
1
Identifiers
DOI: 10.1186/1471-213x-8-43
Keywords
  • Research Article

Abstract

1471-213X-8-43.fm ral ss BioMed CentBMC Developmental Biology Open AcceResearch article Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics Kerstin Buttler1, Taichi Ezaki2 and Jörg Wilting*1,3 Address: 1Centre of Anatomy, Department of Anatomy and Cell Biology, University Medicine Goettingen, Goettingen, Germany, 2Department of Anatomy and Developmental Biology, Tokyo Women's Medical University, Tokyo, Japan and 3University Medicine Goettingen, Centre of Anatomy, Department of Anatomy and Cell Biology, Kreuzbergring 36, 37075 Goettingen, Germany Email: Kerstin Buttler - [email protected]; Taichi Ezaki - [email protected]; Jörg Wilting* - [email protected] * Corresponding author Abstract Background: The data on the embryonic origin of lymphatic endothelial cells (LECs) from either deep embryonic veins or mesenchymal (or circulating) lymphangioblasts presently available remain inconsistent. In various vertebrates, markers for LECs are first expressed in specific segments of embryonic veins arguing for a venous origin of lymph vessels. Very recently, studies on the mouse have strongly supported this view. However, in the chick, we have observed a dual origin of LECs from veins and from mesodermal lymphangioblasts. Additionally, in murine embryos we have detected mesenchymal cells that co-express LEC markers and the pan-leukocyte marker CD45. Here, we have characterized the mesoderm of murine embryos with LEC markers Prox1, Lyve-1 and LA102 in combination with macrophage markers CD11b and F4/80. Results: We observed cells co-expressing both types of markers (e.g. Prox1 – Lyve-1 – F4/80 triple-positive) located in the mesoderm, immediately adjacent to, and within lymph vessels. Our proliferation studies with Ki-67 antibodies showed high proliferative capacities of both the Lyve-1- positive LECs of lymph sacs/lymphatic sprouts and the Lyve-1-positive mesenchymal cells. C

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