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Improvement of chemical analysis of antibiotics:IV. Fluorodensitometric determination of polyether antibiotics

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
295
Identifiers
DOI: 10.1016/s0021-9673(01)87647-0

Abstract

Abstract A fluorodensitometric determination of the polyether antibiotics, salinomycin and monensin, on silica gel thin-layer plates based on fluorescence labelling of carboxylate with 1-bromoacetylpyrene has been established. The optimum conditions for producing salinomycin 1-pyrenacyl ester are described. Using Kryptofix 222 as a catalyst, the reaction fluorescence yield is significantly higher than using dicyclohexyl-18-crown-6. There is a linear relationship between the fluorescence intensity and the amount of salinomycin and monensin between 2 and 14 ng and the detection limits are 100 pg.

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