The ultrastructure of Dentalium gametes, and their fate at fertilization, were investigated. Unexpectedly, fixation carried out in 2% osmium tetroxide, at 4°C, although destroying most cytoplasmic organelles, reveals the presence of bundles of “stress fibers” in the microvilli and the fertilization cone of the eggs. Anti-actin labeling supports the view that the bundles are made of actin. Fertilization, as in other molluses, does not cause any cortical granule exocytosis, at least during the first 15 min following insemination. Ultrastructure of the unreacted and reacted spermatozoon is presented and a tentative model for the morphological interpretation of the acrosomal reaction is proposed. A reevaluation of cytoplasmic heterogeneity, e.g., the so-called cytoplasmic prelocalization, as observed after the germinal vesicle breakdown is also provided as well as an analysis of concomitant surface changes.