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Poly(rI) more important than poly(rC) in the interferon induction process by poly(rI)·poly(rC)

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
54
Issue
1
Identifiers
DOI: 10.1016/0042-6822(73)90137-2

Abstract

Abstract A significantly greater interferon production has been obtained in primary rabbit kidney cell cultures exposed to poly(rI) followed by poly(rC) than in cell cultures exposed to poly(rC) followed by poly(rI). The interferon response in cell cultures exposed to poly(rI) followed by poly(rC) was markedly more resistant to poly- l-lysine and pancreatic ribonuclease treatment than was the interferon response in cell cultures exposed to poly(rC) followed by poly(rI). In addition, poly- l-lysine treatment removed a substantially greater proportion of cell-associated radioactivity from cells exposed to [ 3H]poly(rC) followed by poly(rI) than from cells exposed to poly(rI) followed by [ 3H]poly(rC). These findings suggest that the poly(rI) · poly(rC) complex is more tightly and efficiently bound to the cell (surface) when the homopolymers are added in the order poly(rI), poly(rC) than when they are added in the order poly(rC), poly(rI) and that it is more effectively attached to the cell receptor site by its poly(rI) strand rather than by its poly(rC) strand.

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