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Identification and quantification of insect juvenile hormones as their 10-heptafluorobutyryloxy-11-methoxy derivatives by combination of high-performance liquid chromatography and electron capture gas-liquid chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
170
Issue
1
Identifiers
DOI: 10.1016/s0021-9673(00)84249-1
Disciplines
  • Biology

Abstract

Abstract A convenient procedure is described for the isolation and quantitative determination of juvenile hormones from biological material by their conversion into the 10-heptafluorobutyryloxy-11-methoxy derivatives. After precipitation at low temperature, the lipid is separated by thin-layer chromatography (TLC), and the isolated hormone fraction is converted into the 10-hydroxy-11-methoxy derivatives which are purified by high-performance liquid chromatography (HPLC). These derivatives are treated with heptafluorobutyrylimidazole and the resulting derivatives are finally separated and quantified by electron capture gas-liquid chromatography. Overall yields are 20–50% with 1–4 g insects extracted. Marker substances used are triphenylmethanol for HPLC, and bis(ethylhexyl) phthalate and dibutyl phthalate for TLC and HPLC.

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