Affordable Access

Publisher Website

A new revision of the sequence of plasmid pBR322

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
70
Issue
2
Identifiers
DOI: 10.1016/0378-1119(88)90212-0
Keywords
  • Plasmid
  • Ropgene
  • Expression Vector
  • Translation Initiation
  • Sequence Correction
  • Conserved Sequences
  • Pmbl

Abstract

Abstract A revised sequence in the region immediately upstream from the rop gene of pBR322 is reported. Two base pairs in the accepted sequence do not exist in the plasmid DNA. Specifically, a TA base pair is missing at sequence coordinate 1893 [Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43 (1979) 77–90] and an AT base pair is missing at position 1915, giving a total size for pBR322 of 4361 bp. These changes are in a potential translation initiation sequence and probably reflect errors in the original sequence rather than recent evolution of the plasmid.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

The plasmid, pBR322.

on Biotechnology (Reading, Mass.) 1988

DNA fusion product of phage P2 with plasmid pBR322...

on MGG Molecular & General Geneti... 1983
More articles like this..