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Nonapeptide Hormones Oxytocin and Vasopressin Distinctly Regulate Cav1.2 L-type Calcium Channel Expression in Cardiomyocytes

Authors
Journal
Journal of Arrhythmia
1880-4276
Publisher
Elsevier
Volume
26
Issue
2
Identifiers
DOI: 10.1016/s1880-4276(10)80015-8
Keywords
  • Neurohypophyseal Hormones
  • Gene Transcription
  • Creb
Disciplines
  • Biology
  • Design

Abstract

Background: The neurohypophyseal hormones oxytocin (OT) and [Arg8]-vasopressin (AVP) have recently been implicated in cardiac function. This study was designed to investigate actions of OT and AVP on regulation of the voltage-gated Ca2+ channel in cardiomyocytes. Methods and Results: Cultured neonatal rat cardiomyocytes were stimulated with OT or AVP (10-8 M to 10-6 M) for 24-72 h, followed by real-time PCR and patch clamp studies. Although OT did not exert any modulatory effect on L-type Ca2+ channel current, as a shortterm effect, stimulation of cardiomyocytes with OT but not AVP decreased the expression of Cav1.2 mRNA with a reduced L-type Ca2+ channel current. A transcription factor CREB mRNA expression was down-regulated by OT treatment, although T-type Ca2+ channels (Cav3.1, Cav3.2) were unaffected by OT or AVP. Conclusion: OT but not AVP down-regulates L-type Ca2+ channel expression through the inhibition of CREB transcriptional, suggesting a novel mechanism of OT action in the cardiac electrophysiology.

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