Affordable Access

Publisher Website

Activity of Human Immunodeficiency Virus Type 1 Promoter/TAR Regions andtat1 Genes Derived from Individuals with Different Rates of Disease Progression

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
232
Issue
2
Identifiers
DOI: 10.1006/viro.1997.8586
Disciplines
  • Medicine

Abstract

Abstract Different rates of disease progression may be associated with different human immunodeficiency virus type 1 (HIV-1) promoter and/or transactivator activities. We therefore analyzed the sequences and activities of the first exon of Tat, tat1, and the promoter/ trans-acting responsive (TAR) regions amplified directly from peripheral blood mononuclear cells obtained from five long-term nonprogressors and eight progressing HIV-1-infected individuals. The majority of tat1 alleles and promoter/TAR regions from all patients were intact and showed comparable activities in transient reporter assays. A substantial number of point mutations and some length variations were observed in the promoter/TAR region. In a single nonprogressor, the Sp1 binding site 3 was consistently altered and the transcriptional activity in the presence of Tat was diminished. Some LTR clones from a rapid progressor contained a fourth Sp1 binding site, which was associated with an elevated basal promoter activity. These data suggest that defects in the promoter/TAR region or tat1 are rare and that different promoter/transactivator activities are not commonly associated with different progression rates.

There are no comments yet on this publication. Be the first to share your thoughts.