Affordable Access

Publisher Website

Inhibition of HIV-1 rgp120 binding to CD4+T cells by monoclonal antibodies directed against the gp120 C1 or C4 region

Authors
Journal
Immunology Letters
0165-2478
Publisher
Elsevier
Publication Date
Volume
63
Issue
1
Identifiers
DOI: 10.1016/s0165-2478(98)00047-9
Keywords
  • Hiv-1 Anti-Gp120 Mabs
  • Blockage Of Gp120/Cd4+T Cell Interaction
  • Flow Cytometry
  • Elisa
  • Surface Plasmon Resonance
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Reagents which block the interaction of HIV-1 gp120 with CD4 + T cell are of therapeutic interest. We assessed the ability of the murine monoclonal antibody (mAb) 87-135/9 to bind to the rgp120 C1 region and to block the CD4 interaction, and compared this with the reactivity of the rat mAb 388/389 or the human mAbs F105 and b12, which are known to bind within or near the gp120 C4 region. ELISA and surface plasmon resonance measurements showed that mAb 87-135/9 recognized specifically the gp120 C1 peptide HEDIISLWDQSLK (residues 105–117). All four mAbs bound to rgp120 and blocked its interaction with CD4 + T cells. When mAb 87-135/9 was used in combination with one of the other antibodies, its inhibitory effect was additive. A therapeutic approach could be to use a human anti-gp120/CD4bs conformational mAb in combination with a humanized Ab directed against the conserved, linear gp120 C1 epitope and/or an anti-viral drug to hinder the HIV-1 virus and shedded envelope protein to bind to CD4 + T cells.

There are no comments yet on this publication. Be the first to share your thoughts.