Affordable Access

Publisher Website

Gender and age related expression ofOct-6– a POU III domain transcription factor, in the adult mouse brain

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
344
Issue
2
Identifiers
DOI: 10.1016/s0304-3940(03)00445-2
Keywords
  • Mouse
  • Oct6
  • In Situ Hybridisation
  • Cerebral Cortex
  • Hippocampus
  • Cerebellum
Disciplines
  • Biology

Abstract

Abstract Oct-6 is a POU III domain transcription factor whose primary role is thought to be developmental. It is expressed in embryonic stem cells, Schwann cells, and in neuronal subpopulations during telencephalic development. Its best characterised role is in Schwann cells where it is thought to regulate myelin specific gene expression. Expression of Oct-6 was recently discovered in neurons in post-mortem human schizophrenic specimens while being undetectable in matched controls. This study of human tissue contrasted in a number of regards with earlier studies of rodent brain, and questioned what we can consider to be normal adult expression of this gene. In this study, we have investigated Oct-6 expression via in situ hybridisation and Western blot analysis in normal adult female mice of different ages. We show that both RNA and protein levels of Oct-6 expression are highly sustained in the adult and aging cerebellum, whereas they are attenuated in the telencephalon by PW30 (postnatal week 30). These observations suggest that Oct-6 expression takes place in a sex and age dependent way.

There are no comments yet on this publication. Be the first to share your thoughts.