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Buffers for the reconstitution of aspartate aminotransferase

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
151
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(88)80336-x
Keywords
  • -P
  • Ada
  • Bis-Tris
  • Bis-Tris Propane
Disciplines
  • Biology

Abstract

The cofactor activation of the apoenzyme of pig heart cytosolic aspartate aminotransferase was studied in various buffers. Cationic buffers are shown to allow maximal reconstitution in the pH range of 5.0 to 9.0. Anionic buffers made up of mono- and dicarboxylates are found to affect reconstitution in a pH-dependent manner. At low pH, the carboxylates strongly inhibit reconstitution, but at high pH, they show less effect. In constrast, the more potent inhibitor P i shows the opposite pH profile. Dicarboxylates are considerably more inhibitory than monocarboxylates. Substantial protection against inhibition by a number of carboxylates may be achieved by the addition of sodium chloride.

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