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Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
26
Identifiers
DOI: 10.1016/0378-1119(83)90187-7
Keywords
  • Recombinant Dna
  • Bacteriophage λ
  • Multiple Restriction Sites
  • Polylinkers
  • E. Coli Reca−Hosts

Abstract

Abstract New phage λ-based cloning vectors, Charons 32, 33, 34 and 35, have been constructed. These vectors allow cloning of large (19–21 kb) DNA fragments in up to six cloning sites. DNA cloned in these vectors can be propagated on recA − Escherichia coli hosts

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