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Tissue-specific differences in cultured human diploid fibroblasts

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
108
Issue
1
Identifiers
DOI: 10.1016/s0014-4827(77)80002-5
Disciplines
  • Biology

Abstract

Summary Examination of skin and lung fibroblasts derived from the same human fetuses revealed significant in vitro differences. Fetal lung cultures had faster cell replication rates, greater [ 3H]thymidine incorporation into DNA, higher cell numbers at confluency, smaller cell volumes, decreased cellular RNA and protein contents and lengthened in vitro life spans when compared with fetal skin cultures. In addition, these cell cultures had different responses to the addition of hydrocortisone to culture media. These findings indicate that, despite their morphological similarities, fibroblasts derived from diverse tissues may manifest significant differences in their behavior in tissue culture.

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