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Tissue and subcellular distribution of CLIC1

Authors
Publisher
BioMed Central
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

1471-2121-8-8.fm ral ss BioMed CentBMC Cell Biology Open AcceResearch article Tissue and subcellular distribution of CLIC1 Barbara Ulmasov1, Jonathan Bruno2, Philip G Woost3 and John C Edwards*2 Address: 1Department of Internal Medicine, St. Louis University, St. Louis MO, USA, 2UNC Kidney Center and the Division of Nephrology and Hypertension, Department of Medicine, University of North Carolina, Chapel Hill NC, USA and 3Department of Physiology and Biophysics, Case Western Reserve University, Cleveland OH, USA Email: Barbara Ulmasov - [email protected]; Jonathan Bruno - [email protected]; Philip G Woost - [email protected]; John C Edwards* - [email protected] * Corresponding author Abstract Background: CLIC1 is a chloride channel whose cellular role remains uncertain. The distribution of CLIC1 in normal tissues is largely unknown and conflicting data have been reported regarding the cellular membrane fraction in which CLIC1 resides. Results: New antisera to CLIC1 were generated and were found to be sensitive and specific for detecting this protein. These antisera were used to investigate the distribution of CLIC1 in mouse tissue sections and three cultured cell lines. We find CLIC1 is expressed in the apical domains of several simple columnar epithelia including glandular stomach, small intestine, colon, bile ducts, pancreatic ducts, airway, and the tail of the epididymis, in addition to the previously reported renal proximal tubule. CLIC1 is expressed in a non-polarized distribution in the basal epithelial cell layer of the stratified squamous epithelium of the upper gastrointesitinal tract and the basal cells of the epididymis, and is present diffusely in skeletal muscle. Distribution of CLIC1 was examined in Panc1 cells, a relatively undifferentiated, non-polarized human cell line derived from pancreatic cancer, and T84 cells, a human colon cancer cell line which can form a polarized epithelium that is capable of regulated chloride transport. Digitonin e

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