Affordable Access

Publisher Website

Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize withKlebsiella pneumoniae

Authors
Journal
Microbial Pathogenesis
0882-4010
Publisher
Elsevier
Publication Date
Volume
6
Issue
4
Identifiers
DOI: 10.1016/0882-4010(89)90101-0
Keywords
  • Oligonucleotide Probe
  • Colony Hybridization
  • Ankylosing Spondylitis
  • Klebsiella Pneumoniae
  • Genomic Library
  • Molecular Mimicry
Disciplines
  • Biology

Abstract

Abstract Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66–74 of the HLA-B27.1 sequence and one corresponding to residues 66–74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb Pstl restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.

There are no comments yet on this publication. Be the first to share your thoughts.