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Blood profile during and after hemoglobin substitute administration.

Authors
Publication Date
Keywords
  • Analysis Of Variance
  • Animals
  • Aspirin/Analogs & Derivatives
  • Aspirin/Pharmacology
  • Blood Cell Count
  • Blood Substitutes/Pharmacology
  • Cardiopulmonary Bypass
  • Cattle
  • Hemoglobins/Pharmacology
  • Methemoglobinemia/Etiology
  • Osmolar Concentration
Disciplines
  • Chemistry
  • Medicine

Abstract

The in vivo effects of Diaspirin Crosslinked Hemoglobin (DCLHb, Baxter Healthcare Corp.) on hematology and biochemistry are unknown. This study includes 6 calves (71.2+/-1.3 kg). In each animal a total of 2 litres of blood was exchanged for the same amount of hydroxylethyl starch (Haes, Fresenius) (n=3) or DCLHb (n=3), which is equivalent to 28cc/kg of blood substitute, over a period of 5 hours. The animals were allowed to survive 7 days. Blood samples were taken hourly during the perfusion protocol, at postoperative day (POD) 1, 2 and 7. ANOVA test was used for repeated measurements. Blood cell profiles were similar in both groups. Peak methemoglobinemia was 4.2% in the DCLHb group. Osmolarity was significantly higher in the DCLHb group with the greatest difference at POD 1 and 2. Postmortem analysis of the major organs did not show any sign of hemoglobin deposit in the DCLHb group. In the given setup DCLHb can be administered in a large quantity with good hematological tolerance and without any deposits in major organs. A prolonged plasma expander effect was observed.

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