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The Author Response: EML4-ALK Fusion Gene in Korean Non-Small Cell Lung Cancer

Journal of Korean Medical Science
Korean Academy of Medical Sciences (KAMJE)
Publication Date
DOI: 10.3346/jkms.2012.27.5.578
  • Correspondence
  • Chemistry
  • Design


© 2012 The Korean Academy of Medical Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. pISSN 1011-8934 eISSN 1598-6357 • J Korean Med Sci 2012; 27: 578 CORRESPONDENCE The Author Response EML4-ALK Fusion Gene in Korean Non-Small Cell Lung Cancer Jae Yong Park Department of Biochemistry and Cell Biology, and Internal Medicine, Kyungpook National University School of Medicine, Daegu, Korea I would like to thank the interest and comments on our paper entitled “EML4-ALK fusion gene in Korean non-small cell lung cancer” (1). In this study, we examined EML4-ALK fusion vari- ants in Korean non-small cell lung cancers (NSCLCs) via reverse- transcriptase-polymerase chain reaction (RT-PCR) using prim- ers designed to detect EML4-ALK fusion variants (variants 1, 2, 3a, 3b, 4, 5a, 5b, 6, and 7) that have been previously identified (2, 3). Our study demonstrated the spectrum and frequency of EML4-ALK fusion variants in Korean NSCLCs, which were dif- ferent from those in other ethnic populations. I agree with the comment that the RT-PCR technology for iden- tification of ALK fusion variants has several limitations. As point- ed out in this comment, there are multiple EML4-ALK fusion variants and non-EML4 fusion partners, such as KIF5B, and KLC1 (2-5); therefore, any PCR-based strategy must incorpo- rate validated primer pairs for all known ALK fusions. Another limitation is that given that most specimens from lung cancer patients are stored as formalin-fixed paraffin embedded tissue, the RNA may have been substantially degraded relative to non- fixed, fresh-frozen tissue. In addition, it has been reported that PCR-based detection of EML4-ALK can yield positive results in th

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