Abstract An anorectic substance similar to satietin was purified from ethanolic-acid precipitate of rat urine by gel filtration and ion exchange chromatography. The obtained glycoprotein (molecular mass 67 kDa) reduced food intake after intraperitoneal and intracisternal administration in mice in a dose-dependent manner (in the range of 5–80 mg/kg). The anorectic effect was specific. In the presence of sodium dodecylsulphate the substance dissociated into small proteins with a molecular mass of 9–11 kDa. Its anorectic effect was long lasting and differed from that of the anorectic peptide cholecystokinin octapeptide. Our finding supports the idea that anorectic protein isolated from plasma, membranes, and urine may be related substances.