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A quenched-flow apparatus which allows the measurement of the kinetics of a reaction in one stroke

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
9
Issue
3
Identifiers
DOI: 10.1016/0165-022x(84)90025-3
Keywords
  • Quenched Flow Apparatus
  • Reaction Kinetics
Disciplines
  • Chemistry

Abstract

Abstract A chemical quenched-flow apparatus is described which measures, in a unique stroke, enough data points (8–11) for establishing the kinetics curve of a reaction. Only very small volumes of reaction solutions (2 × 500 μl) are required. The time intervals between which the kinetic data may be measured range from 5 to 37 ms and from 120 to 450 ms with the corresponding mixing times of 0.6 and 5 ms, respectively. This apparatus was used to investigate the pre-steady-state domain of the aminoacylation reaction of tRNA Val by valyl-tRNA synthetase from yeast.

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