Affordable Access

Publisher Website

An improved method for correlative light and electron microscopic examination of cobaltic-lysine-labelled neurons

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
73
Issue
2
Identifiers
DOI: 10.1016/0304-3940(87)90015-2
Keywords
  • Cobaltic-Lysine Complex
  • Electron Microscopy
  • Light Microscopy
  • Synaptic Organization
  • Axonal Transport
  • Extracellular Labelling

Abstract

Abstract We describe an improved method for correlative light and electron microscopic examination of synaptic organization of neurons extracellularly labelled by the axonal transport of cobaltic-lysine (Co-lys). After filling the neurons with Co-lys and precipitating the CoS, the brain is fixed in a double-aldehyde fixative, and thick slices are cut using a Microslicer. The slices are intensified using a physical developer, postfixed in OsO 4 and embedded in resin. By cutting alternating semithin and ultrathin sections, it is possible to specify sites and types of synaptic contacts on labelled neuronal profiles.

There are no comments yet on this publication. Be the first to share your thoughts.