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Dry-coated microprojection array patches for targeted delivery of immunotherapeutics to the skin

Authors
Journal
Journal of Controlled Release
0168-3659
Publisher
Elsevier
Publication Date
Volume
139
Issue
3
Identifiers
DOI: 10.1016/j.jconrel.2009.06.029
Keywords
  • Microprojections
  • Gas-Jet Drying Coating
  • Targeted Vaccine Delivery
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Dry-coated microprojections (MPs) deliver vaccine to abundant immunogenic cells within the skin to induce immune responses. Success in this targeted vaccine delivery relies on overcoming the challenges of dry-coating the vaccine onto the very small (≤ 90 µm length) and densely packed (~ 20,000 cm − 2 ) MPs. In this paper, we show that a gas-jet drying coating method achieves the desired uniform coating. The coating approach is robustly demonstrated on compounds representative of a range of immunotherapeutics (e.g. DNA, proteins), with each uniformly coated on thousands of MPs. Furthermore, the dry-coating remains intact during skin insertion, and then releases within the wet skin cellular environment within 3 min. Finally, we applied ovalbumin protein coated MP patches to immunise mice, achieving comparable antibody levels ( p = 0.08) with needle and syringe intramuscular injection. In summary, this paper presents a simple, practical and versatile method to achieve uniform coating on very small and densely packed MPs for a needle-free and targeted vaccine delivery technology.

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