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Highly sensitive SERS detection of cancer proteins in low sample volume using hollow core photonic crystal fiber

Authors
Journal
Biosensors and Bioelectronics
0956-5663
Publisher
Elsevier
Publication Date
Volume
33
Issue
1
Identifiers
DOI: 10.1016/j.bios.2011.12.056
Keywords
  • Surface Enhanced Raman Scattering
  • Photonic Crystal Fiber
  • Sers Nanotag
  • Protein Sensing
  • Bioconjugation
Disciplines
  • Biology
  • Medicine
  • Physics

Abstract

Abstract Enzyme-linked immunosorbent assays (ELISA) are commonly used for detecting cancer proteins at concentration in the range of about ng-μg/mL. Hence it often fails to detect tumor markers at the early stages of cancer and other diseases where the amount of protein is extremely low. Herein, we report a novel photonic crystal fiber (PCF) based surface enhanced Raman scattering (SERS) sensing platform for the ultrasensitive detection of cancer proteins in an extremely low sample volume. As a proof of concept, epidermal growth factor receptors (EGFRs) in a lysate solution from human epithelial carcinoma cells were immobilized into the hollow core PCF. Highly sensitive detection of protein was achieved using anti-EGFR antibody conjugated SERS nanotag. This SERS nanotag probe was realized by anchoring highly active Raman molecules onto the gold nanoparticles followed by bioconjugation. The proposed sensing method can detect low amount of proteins at ∼100pg in a sample volume of ∼10nL. Our approach may lead to the highly sensitive protein sensing methodology for the early detection of diseases.

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