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Cloning of eukaryotic genes in single-strand phage vectors: the human interferon genes

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
27
Issue
1
Identifiers
DOI: 10.1016/0378-1119(84)90241-5
Keywords
  • Recombinant Dna
  • Bacteriophage F1
  • Leukocyte
  • Fibroblast
  • Lacuv5 Promoter
  • Escherichia Colihost
  • Cdna
  • Dna Sequencing
Disciplines
  • Biology

Abstract

Abstract Using oligonucleotide probes with defined sequences, we have selected clones from a human lymphocyte cDNA library which represent human leukocyte (HuIFN-α) and fibroblast (HuIFN-β) interferon gene sequences. Double-stranded f1 phage DNA was used as the vector for initial cloning of cDNA. Clones carrying interferon gene sequences were identified by hybridization with the oligonucleotide probes. The same oligonucleotide probes were used as primers for dideoxy chain termination sequencing of the clones. One HuIFN-α clone, 201, has a nucleotide sequence different from published HuIFN-α sequences. Under control of the lacUV5 promoter, the 201 gene has been used to express biologically active HuIFN-α in Escherichia coli.

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